Description
Arf1(Δ17Q71L) 蛋白
货号: 10123 |
描述: Arf1 Protein Δ17 Q71L mutant |
基因符号: ADP-ribosylation factor 1 |
背景介绍: Arf1 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. |
氨基酸序列 (1-181, Δ17, Q71L) MGNIFANLFKGLFGKK-MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWD VGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK |
来源: Human recombinant, His6-tag |
表达宿主: E. coli |
分子量 21 kDa |
纯度: >95% by SDS-PAGE |
成分: Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
物理外观: White or clear |
浓度: 1 mg/mL |
贮存: -80°C |
制备方法: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf1 Δ17Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining |
References:
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