Description
Arf6(Δ12Q67L) 蛋白
| 货号: 10125 |
| 描述: Arf6 Protein Δ12Q67L mutant |
| 基因符号: ADP-ribosylation factor 6 |
| 背景介绍: Arf6 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and the actin cytoskeketon at the plasma membrane and functions as a regulatory molecule of phagocytosis. |
| 氨基酸序列 (1-175, Δ12, Q67L) MGKVLSKIFGN-EMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGL DKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHE IQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS |
| 来源: Human, recombinant, His6-tag |
| 表达宿主: E. coli |
| 分子量 20 kDa |
| 纯度: >95% by SDS-PAGE |
| 成分: Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| 物理外观: White or clear |
| 浓度: 1mg/mL |
| 贮存: -80°C |
| 制备方法: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf6 Δ12Q67L mutant was determined by SDS-PAGE and Coomassie Brilliant Blue Staining. |
References:
1. Cavenagh, M. M. et al., J. Biol. Chem. 271: 21767-21774, 1996.
2. D’Souza-Schorey, C. et al., Science 267: 1175-1178, 1995.
3. Falace, A. et al., Am. J. Hum. Genet. 87: 365-370, 2010.
4. Hernandez-Deviez, D. J. et al., Nature Neurosci. 5: 623-624, 2002.
5. O’Neal, C. J. et al., Science 309: 1093-1096, 2005.
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