Description
BRAF(G469E) 单抗
| 货号: 26196 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRAF(G469E) Mouse Monoclonal Antibody |
| 背景介绍: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| 免疫原: A synthetic peptide from the internal region of BRAF , which includes the mutation of G469E, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 IHC: 1: 50-1: 100 |
| 浓度: 1mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes G469E mutant, but not wild-type BRAF of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
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蛋白质印迹: Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti-BRAF(G469E) monoclonal antibody (cat. # 26196). 免疫荧光:
Immunofluorescence of cells expressing BRAF proteins with Anti-BRAF(G469E) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with Anti-BRAF(G469E) monoclonal antibody (cat. # 26196). |
Western blot analysis of recombinant BRAF (G469E) and wildtype proteins.
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