Description
BRCA1(C61G) 单抗
| 货号: 26319 |
| 基因符号: BRCA1, BRCC1, IRIS, PNCA4, PPP1R53, PSCP |
| 描述: Anti-BRCA1(C61G) Mouse Monoclonal Antibody |
| 背景介绍: BRCA1 is normally expressed in cells of breast and other tissues, where it helps repair damaged DNA or destroy cells if DNA cannot be repaired. It is involved in the repair of chromosomal damage with an important role in the error-free repair of DNA double-strand breaks. If BRCA1 itself is damaged by a BRCA1 mutation, damaged DNA is not repaired properly, and this increases the risk for breast cancer. |
| 免疫原: A synthetic peptide from the internal region of BRCA1, which includes the mutation of C61G, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 2000 WB: 1: 500-1: 1000 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes C61G mutant, but not wild-type BRCA1 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
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蛋白质印迹: Western blot analysis of recombinant BRCA1(C61G) and wildtype proteins. Purified His-tagged BRCA1(C61G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti-BRCA1(C61G) monoclonal antibody (Cat. #26319). 免疫荧光:
Immunofluorescence of cells expressing BRCA1 proteins with Anti-BRCA1(C61G) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRCA1 (WT) plasmid (left column) or pCDNA3-GFP-BRCA1(C61G) plasmid (right column), then fixed and stained with Anti-BRCA1(C61G) monoclonal antibody (Cat. #26319). |
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