Description
CTCF(K344E) 单抗
货号: 26324 |
基因符号: CTCF; MRD21 |
描述: Anti-CTCF(K344E) Mouse Monoclonal Antibody |
背景介绍: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture. |
免疫原: A synthetic peptide from the internal region of CTCF which includes the mutation of K344E, human origin |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 IHC: 1: 50-1: 100 |
浓度: 1 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes K344E mutant, but not wild-type CTCF of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Purified His-tagged CTCF (K344E) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti-CTCF(K344E) monoclonal antibody (Cat. #26324). 免疫荧光: HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (K344E) plasmid (right column), then fixed and stained with Anti-CTCF(K344E) monoclonal antibody (Cat. #26324). |
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