Description
CTNNB1(T41A) 单抗
| 货号: 26305 |
| 基因符号: Beta-catenin, CTNNB, Catenin beta-1 |
| 描述: Anti-CTNNB1(T41A) Mouse Monoclonal Antibody |
| 背景介绍: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherent junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers. |
| 免疫原: A synthetic peptide from the internal region of CTNNB1, which includes the mutation of T41A, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 2000 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes T41A mutant, but not wild-type CTNNB1 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:  Western blot analysis of recombinant CTNNB1 (T41A) and wildtype proteins. Purified His-tagged CTNNB1 (T41A) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti-CTNNB1(T41A) monoclonal antibody (Cat. #26305). 免疫荧光:  Immunofluorescence of cells expressing CTNNB1 proteins with Anti-CTNNB1(T41A) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (T41A) plasmid (right column), then fixed and stained with Anti-CTNNB1(T41A) monoclonal antibody (Cat. #26305). |
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