HRAS(G13A) 小鼠单抗

Description

Ras(G13A)

货号: 26191

基因符号: H-Ras; K-Ras; N-Ras

描述: Anti-HRAS(G13A) Mouse Monoclonal Antibody

背景介绍: Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.

免疫原: A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin

经过测试的应用: ELISA, WB, IHC

推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 500-1: 1000
IHC: 1: 50-1: 100

浓度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亚型: IgG

纯度: Purified from ascites

防腐剂: No

成分: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: Recognizes G13A mutant, but not wild-type H-RAS of vertebrates

储存条件: Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:

Western blot analysis of recombinant HRAS(G13A) and wildtype proteins.

Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti-HRAS(G13A) monoclonal antibody (Cat. #26191).
免疫荧光:

Immunofluorescence of cells expressing Ras proteins with Anti-HRAS(G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRAS(G13A) plasmid (right column), then fixed and stained with Anti-HRAS(G13A) monoclonal antibody (Cat. #26191).

Additional information

品牌	NewEast Biosciences

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