Description
Rab7(Q67L) 突变蛋白
| 货号: 10135 |
| 描述: Rab7 Protein Q67L mutant |
| 基因符号: Member RAS oncogene family, Rab7a, MGC102153 |
| 背景介绍: Members of the Rab family of Ras-related GTP-binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. Rab7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. |
| 氨基酸序列 (1-207, Q67L) MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGL ERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQV ATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAES CSC |
| 来源: Human, recombinant full length, His6-tag |
| 表达宿主: E. coli |
| 分子量 23 kDa |
| 纯度: >95% by SDS-PAGE |
| 成分: Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| 物理外观: White or clear |
| 浓度: 1 mg/mL |
| 贮存: -80°C |
| 制备方法: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab7 Q67L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining. |
References:
1. Edinger, A. L. et al., Dev. Cell 5: 571-582, 2003. 2. Houlden, H. et al., Ann. Neurol. 56: 586-590, 2004.
3. Meggouh, F. et al., Neurology 67: 1476-1478, 2006.
4. Rak, A. et al., Cell 117: 749-760, 2004.
5. Verhoeven, K. et al., Am. J. Hum. Genet. 72: 722-727, 2003.
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