Description
Ran 蛋白
| 货号: 10112 |
| 描述: Ran Protein |
| 基因符号: Ras-related nuclear protein, TC4, Gsp1, ARA24 |
| 背景介绍: Ran is a member of the Ras-superfamily GTPases. Ran is involved in control of DNA synthesis and of cell cycle progression, and the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. |
| 氨基酸序列 (1-216) MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTA GQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAK SIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLE VAQTTALPDEDDDL |
| 来源: Human, recombinant full length, His6-tag |
| 表达宿主: E. coli |
| 分子量 24 kDa |
| 纯度: >95% by SDS-PAGE |
| 成分: Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| 物理外观: White or clear |
| 浓度: 1 mg/mL |
| 贮存: -80°C |
| 制备方法: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Ran was determined by SDS- PAGE and Coomassie Brilliant Blue Staining. |
References:
1. Carazo-Salas, R. E. et al., Nature 400: 178-181, 1999.
2. Caudron, M. et al., Science 309: 1373-1376, 2005.
3. Kalab, P. et al., Nature 440: 697-701, 2006.
4. Lee, S. J. et al., Nature 435: 693-696, 2005.
5. Monecke, T. et al., Science 324: 1087-1091, 2009.
6. Ohba, T. et al., Science 284: 1356-1358, 1999.
7. Seewald, M. J. et al., Nature 415: 662-666, 2002.
8. Smith, A. E. et al., Science 295: 488-491, 2002.
9. Walther, T. C. et al., Nature 424: 689-694, 2003.
10. Wiese, C. et al., Science 291: 653-656, 2001.
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